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Université de Lille

Technical platforms & equipments

Our technical platforms are an essential lever for improving the organization of laboratories and research structures. By pooling measuring instruments, spaces, and expertise, they enhance organizational performance while meeting the objectives of scientific relevance and quality. This structure promotes a more rational use of resources and limits redundancy. The centralization of technical resources also contributes to strengthening the reliability of our results and the overall quality of the work performed.

The effectiveness of our technical platforms is also based on a structure centered around technical referents. These experts are responsible for supporting users, monitoring equipment, and ensuring compliance with best practices. They play a key role in knowledge sharing, user training, and maintaining high technical and methodological standards. Beyond the simple sharing of equipment, these platforms foster a collective dynamic of continuous improvement, collaboration, and skill development within the unit.

Technical platforms

Locations:

  • Primary cells : 4th Centre
  • Cell lines & Lentivirus : 5th centre
  • Bacteriology : 5th Centre Est

Main coordinator:

  • Bertrand Meresse - Room E413b

Primary cells coordinator:

  • Marie Delbeke - Room E409
  • Solenne Dezitter - Room E412

Cell lines coordinator:

  • Manel Jendoubi - Room C529
  • Solange Vivier - Room C518

Lentivirus coordinator:

  • Noémie Legrand - Room E4220

Bacteriology coordinator:

  • Valérie Gouyer - Room C507b

Equipments

Histology: Cryostat, microtome, stations for embedding, fixation, staining, and demasking…

Microscopy: Two microscopes, visible light, fluorescence, scanning (DMi8), stereomicroscope

Contacts

Main coordinator : Madjid DJOUINA

Histology coordinator : Christophe WAXIN & Léonie VANDOMBER

Microscopy coordinator : Matthias DANES

Equipment: Heating blocks, migration chambers, generators, semi-dry transfer units, and gel/membrane readers

Coordinators: Alexis LARGY & Solange VIVIER

Equipment: Two Q-PCR instruments, two thermal cyclers, two NanoDrop instruments, and one bioanalyzer

Coordinator: Mathilde BODY-MALAPEL

Techniques:

  • Preparation of tissue sections
  • Sacrification/dissection, injections/gavage
  • Infusion (e.g., preparation of primary hepatocytes)
  • Glucose, pyruvate, and insulin tolerance tests

Coordinator: Ophélie BERTIN

Equipment:

  • PSM and CO₂ incubator
  • Pipetting robot (Hamilton Microlab Prep)
  • Multimode plate reader (BMG ClarioStar PLUS)
  • 96 / 384 wells  - TR-FRET
  • Absorbance  - AlphaScreen
  • Luminescence  - injector
  • Fluorescence

Plate-based assays with up to 384 wells:

  • Assay of cytokines, phosphoproteins, and post-translational modifications
  • Studies of ligand-receptor interactions or protein-protein interactions
  • Studies of enzyme activity, second messengers, etc.

Coordinators: Amélie BARCZYK & Xavier DEZITTER

Coordinator: Manel JENDOUBI

Common facilities outside the platforms

Location: C503

Main coordinator: Jean-Luc DESSEYN

Coordinators: Valérie GOUYER & Léonie VANDOMBER

Localisation: E407

Coordinator: Arnaud DENDOOVEN

Localisation: C530

Coordinator: Rodolphe CARPENTIER

Equipment available within the unit

Contact persons:

References:

  • Legrand et al, PLoS One, 2014.
  • Legrand et al, Nucleic Acids Research, 2015.
  • Legrand et al, Cancers, 2021.
  • Sobolewski et al, CMGH, 2021.
  • Sobolewski C, Dubuquoy L and Legrand N, Cancers, 2022.
  • Sobolewski et al, Cancers, 2022.